Gene description for HSPA8
Gene name heat shock protein family A (Hsp70) member 8
Gene symbol HSPA8
Other names/aliases HEL-33
HEL-S-72p
HSC54
HSC70
HSC71
HSP71
HSP73
HSPA10
LAP-1
LAP1
NIP71
Species Homo sapiens
 Experiment description of studies that identified HSPA8 in Cervical cancer cells

1
Experiment ID1289
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 19912576   
OrganismHomo sapiens
Experiment descriptionHIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells.
AuthorsLenassi M, Cagney G, Liao M, Vaupotic T, Bartholomeeusen K, Cheng Y, Krogan NJ, Plemenitas A, Peterlin BM.
Journal name Traffic.
Publication year2010
SampleCervical cancer cells
Sample nameHeLa.CIITA cells with Nef-GFP
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV100021: EV-METRIC:33%, 25%

2
Experiment ID1607
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD63 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1611
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD9 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

4
Experiment ID1613
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

5
Experiment ID1613
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID1614
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

7
Experiment ID1614
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

8
Experiment ID1615
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

9
Experiment ID1616
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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